|
KMID : 0368419980410040255
|
|
Journal of Plant Biology
1998 Volume.41 No. 4 p.255 ~ p.261
|
|
|
Transgenic Tobacco Plants Expressing the Coat Protein of Cucumber Mosaic Virus Show Different Virus Resistance
|
|
Ryu, Ki Hyun
Lee, Gung Pyo/Park, Kuen Woo/Lee, Se Yong/Park, Won Mok
|
|
|
Abstract
|
|
|
|
Transgenic tobacco (Nicotiana tabacum cv. Xanthi-nc) plants were regenerated after cocultivation of leaf explants with Agrobacterium tumefaciens strain LBA4404 harboring a plasmid that contained the coat protein (CP) gene of cucumber mosaic virus (CMV-As). PCR and Southern blot analyses revealed that the CMV CP gene was successfully introduced into the genomic DNA of the transgenic tobacco plants. Transgenic plants (CP^+) expressing CP were obtained and used for screening the virus resistance. They could be categorized into three types after inoculation with the virus: virus-resistant, delay of symptom development, and susceptible type. Most of the CP^+ transgenic tobacco plants failed to develop symptoms or showed systemic symptom development delayed for 5 to 42 days as compared to those of nontransgenic control plants after challenged with the same virus. However, some CP^+ transgenic plants were highly susceptible after inoculation with the virus. Our results suggest that the CP-mediated viral resistance is readily applicable to CMV disease in other crops.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|